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Nature：科學(xué)家破譯猛犸象80%基因組
來源 新浪科技
Nature：科學(xué)家破譯猛犸象80%基因組   美國科學(xué)家日前通過一團猛犸象的毛發(fā)，成功破譯出這個史前龐然大物80%的基因組。盡管這是一團毫無光澤的毛發(fā)，卻使科學(xué)家在復(fù)活猛犸象的道路上又向前邁進了一步。
   科學(xué)家通過已在西伯利亞長久凍結(jié)帶冷凍數(shù)千年的猛犸象尸體提取的毛發(fā)樣本，整理出這種史前巨獸的DNA?；虼a讓科學(xué)家對猛犸象的進化過程有了新的了解，同時表明它們遠比之前想象的更接近于現(xiàn)代象。這項發(fā)現(xiàn)還可以使研究人員搞清楚大象的遺傳構(gòu)造，復(fù)活滅絕已久的猛犸象。
   科學(xué)家利用從西伯利亞長久凍結(jié)帶發(fā)現(xiàn)的兩具猛犸象殘骸上獲取的DNA，實施了這種類似于科幻大片《侏羅紀公園》劇情的研究。美國科學(xué)家在*新一期科學(xué)雜志《自然》（Nature）上報告稱，在這兩具猛犸象尸體中，一具已在地下埋了2萬年，另一具則至少埋了6萬年。通過仔細分析它們的DNA，研究小組已經(jīng)整理出80%的猛犸象基因組或遺傳密碼。
   這項研究表明，兩頭猛犸象關(guān)系十分緊密。另外分析還表明，600萬年前，猛犸象和現(xiàn)代象走向了不同的進化路徑，幾乎與此同時，人類和黑猩猩也走上了與它們一樣的進化道路。200萬年前，猛犸象一分為二，一組在距今4.5萬年前滅絕，另一組在距今1萬年前*后一個冰河時期的末期從地球上消失。
   雄性猛犸象高約10英尺(約合3米)，雌性相對矮一些。它們的尖牙長而彎曲，腹部的毛發(fā)*長可達3英尺(約合0.9米)。迄今，科學(xué)家已在西伯利亞發(fā)現(xiàn)了大約40具猛犸象的尸體。實施*新研究的美國科學(xué)家之所以使用提取自猛犸象毛發(fā)的DNA，是因為相對于猛犸象尸體其他部位的DNA，它們保存得更為完好。
   一些研究人員一直建議使用冰凍猛犸象尸體的皮膚或毛發(fā)克隆猛犸象。領(lǐng)導(dǎo)這項研究的賓夕法尼亞州立大學(xué)教授史蒂芬?舒斯特(StephanSchuster)說：“從理論上講，通過破譯這個基因組，我們可以獲取重要的信息，將來有**，只要將獨特的猛犸象DNA序列融入現(xiàn)代象的基因組中，這些信息或能幫助其他研究人員復(fù)活猛犸象?！?br>    但是，西澳大利亞默多克大學(xué)古生物DNA實驗室主任邁克爾?邦斯(MichaelBunce)博士給舒斯特教授潑了一瓢涼水。他說：“掌握某種生物的DNA代碼并不意味著我們可以通過遺傳手段實現(xiàn)重造滅絕生物體的美好愿望?！保▌?chuàng)賽新聞中心canspecsci.com）

創(chuàng)賽推薦原始出處：
Nature，456, 387-390，Webb Miller，Stephan C. Schuster
Sequencing the nuclear genome of the extinct woolly mammoth
Webb Miller1, Daniela I. Drautz1, Aakrosh Ratan1, Barbara Pusey1,Ji Qi1, Arthur M. Lesk1, Lynn P. Tomsho1, Michael D. Packard1,Fangqing Zhao1, Andrei Sher2,9, Alexei Tikhonov3, Brian Raney4,Nick Patterson5, Kerstin Lindblad-Toh5, Eric S. Lander5, James R.Knight6, Gerard P. Irzyk6, Karin M. Fredrikson7, Timothy T.Harkins7, Sharon Sheridan7, Tom Pringle8 & Stephan C.Schuster1
1 Pennsylvania State University, Center for Comparative Genomicsand Bioinformatics, 310 Wartik Building, University Park,Pennsylvania 16802, USA
2 Severtsov Institute of Ecology and Evolution, Russian Academy ofSciences, 33 Leninsky Prospect, 119071 Moscow, Russia
3 Zoological Institute, Russian Academy of Sciences,Universitetskaya Naberezhnaya 1, 199034 Saint Petersburg,Russia
4 Center for Biomolecular Science and Engineering, University ofCalifornia, Santa Cruz, California 95064, USA
5 Broad Institute of MIT and Harvard, 7 Cambridge Center,Cambridge, Massachusetts 02142, USA
6 454 Life Sciences, 20 Commercial Street, Branford, Connecticut06405, USA
7 Roche Diagnostics Corporation, 9115 Hague Road, Indianapolis,Indiana 46250-0414, USA
8 Sperling Foundation, Eugene, Oregon 97405, USA
In 1994, two independent groups extracted DNA from severalPleistocene epoch mammoths and noted differences among individualspecimens1, 2. Subsequently, DNA sequences have been published fora number of extinct species. However, such ancient DNA is oftenfragmented and damaged3, and studies to date have typically focusedon short mitochondrial sequences, never yielding more than afraction of a per cent of any nuclear genome. Here we describe 4.17billion bases (Gb) of sequence from several mammoth specimens, 3.3billion (80%) of which are from the woolly mammoth (Mammuthusprimigenius) genome and thus comprise an extensive set ofgenome-wide sequence from an extinct species. Our data supportearlier reports4 that elephantid genomes exceed 4 Gb. The estimateddivergence rate between mammoth and African elephant is half ofthat between human and chimpanzee. The observed number ofnucleotide differences between two particular mammoths wasapproximately one-eighth of that between one of them and theAfrican elephant, corresponding to a separation between themammoths of 1.5–2.0 Myr. The estimated probability that orthologouselephant and mammoth amino acids differ is 0.002, corresponding toabout one residue per protein. Differences were discovered betweenmammoth and African elephant in amino-acid positions that areotherwise invariant over several billion years of combinedmammalian evolution. This study shows that nuclear genomesequencing of extinct species can reveal population differences notevident from the fossil record, and perhaps even discover geneticfactors that affect extinction.
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