細(xì)胞解離產(chǎn)品
產(chǎn)品名稱: 細(xì)胞解離產(chǎn)品
產(chǎn)品型號(hào): C14-13150-016
產(chǎn)品展商: GIBCO
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
細(xì)胞解離液Cell dissociation buffer
不含酶,由Hank’s液配制
保存:15-30°
細(xì)胞解離產(chǎn)品
的詳細(xì)介紹
詳細(xì)說(shuō)明:
Cell Dissociation Buffers are membrane-filtered, isotonic, and enzyme-free aqueous formulations of salts, chelating agents, and cell-conditioning agents in either Ca2+- and Mg2+-free Hanks balanced salt solution or Ca2+- and Mg2+-free phosphate-buffered saline. They are suitable for gentle dissociation of mammalian cells from support substrates and each other, for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry (1,2). These buffers are not recommended for routine passage of strongly adherent cell types.
Performance and quality testing: Buffers are membrane filtered and mycoplasma tested. Cell integrity after dissociation with buffers is measured with a plating efficiency assay and a serotonin receptor function assay.
常用規(guī)格
Chelators: |
EDTA
|
Product Size: |
100 ml
|
Classification: |
Animal Origin-Free
|
Enzyme Stability: |
Room Temperature Stable
|
Phenol Red Indicator: |
No Phenol Red
|
相關(guān)信息:
品名
|
產(chǎn)品描述
|
貨號(hào)
|
規(guī)格
|
細(xì)胞解離液
Cell dissociation buffer
|
不含酶,由Hank’s液配制
保存:15-30°
|
13150-016
|
100ml
|
細(xì)胞解離液
Cell dissociation buffer
|
不含酶,由PBS配制
保存:15-30°
|
13151-014
|
100ml
|
膠原酶,collagenase
|
活性:每毫克不少于125U,2-8°
|
17018-029
|
500mg
|
I型膠原酶,collagenase Type I
|
可解離組織,建議用于上皮、肺、脂肪和腎上腺組織細(xì)胞,2-8°保存
|
17100-017
|
1g
|
II型膠原酶,collagenase Type II
|
可解離組織,建議用于肝、骨、甲狀腺、心和唾液腺組織細(xì)胞,2-8°保存
|
17101-015
|
1g
|
IV型膠原酶,collagenase Type IV
|
可解離胰腺組織,用于分離胰島,
2-8°保存
|
17104-019
|
1g
|
分散酶,Dispase
|
溫和的解離組織得到單個(gè)細(xì)胞,
2-8°保存
|
17105-041
|
5g
|
胰酶替代物,TrypLE Select
|
配方包含1mM EDTA的DPBS,
不含酚紅,保存:2-8°
|
12563-011
|
100ml
|
|
12563-029
|
500ml
|
胰酶替代物,TrypLE Express
|
配方包含1mM的 DPBS,
含酚紅,保存:2-8°
|
12605-010
|
100ml
|
|
12605-028
|
500ml
|
胰酶替代物,TrypLE Express
|
配方包含1mM的DPBS,
不含酚紅,保存:2-8°
|
12604-013
|
100ml
|
|
12604-021
|
500ml
|
胰酶,Trpsin,0.25%
|
不含鈣鎂離子,含酚紅
保存:-5 - -20°
|
15050-065
|
100ml
|
|
15050-057
|
500ml
|
胰酶,Trpsin,2.5%(10X)
|
無(wú)菌條件下以Hank’s液10倍稀釋?zhuān)?/span>
不含鈣鎂離子,保存:-5 - -20°
|
15090-046
|
100ml
|
胰酶-EDTA,
0.05%胰酶,EDTA。4Na
|
不含鈣鎂離子,含酚紅
保存:-5 - -20°
|
25300-054
|
100ml
|
25300-062
|
500ml
|
胰酶-EDTA,(10X)
0. 5%胰酶,EDTA。4Na
|
無(wú)菌條件下以Hank’s液10倍稀釋?zhuān)?/span>
不含鈣鎂離子,保存:-5 - -20°
|
15400-054
|
100ml
|
胰酶-EDTA,
0.25%胰酶,EDTA。4Na
|
不含鈣鎂離子,含酚紅
保存:-5 - -20°
|
25200-056
|
100ml
|
25200-072
|
500ml
|
胰酶(1:250),干粉
|
保存:2-8°
|
27250-018
|
100g
|
胰酶抑制劑,大豆
|
0.25-0.5mg/ml的PBS溶液滅活等體積胰酶,保存:-5 - -20°
|
17075-029
|
1g
|
EDTA,Versene,1:5000
|
0.2g/l EDTA.4Na磷酸緩沖鹽溶液,
=0.48Mm,保存:2-8°
|
15040-066
|
100ml
|
Low Trypsin-High EDTA,
|
0.025% 胰酶, 0.75mM EDTA (1X), 溶于PBS,不含鈣鎂離子
|
SM-2004-C
|
100ml
|
Trypsin 0.25%
|
Hank‘s 液,不含鈣鎂離子
|
SM-2001-C
|
100ml
|
Accutase
|
|
SCR005
|
100ml
|
Accumax
|
|
SCR006
|
100ml
|
Trypsin-EDTA
|
0.05% Trypsin & 0.53mM EDTA,
溶于Hank‘s液,不含鈣鎂
|
SM-2002-C
|
100ml
|
Trypsin-EDTA
|
0.25% Trypsin & 1mM EDTA,
溶于Hank‘s液,不含鈣鎂離子
|
SM-2003-C
|
100ml
|