內(nèi)容和儲存
The Expressway™ Maxi Cell-Free Expression System is designed to perform two hundred 25-µl, five 1-ml, or one 5-ml (initial volume) reactions or one to several large reactions, depending on your needs. This system includes the E.coli slyD- Extract, 2.5X IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, T7 Enzyme Mix, and a positive expression control vector. The Expressway™ Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO® and pEXP5-CT/TOPO® also includes the pEXP5-NT/TOPO® and pEXP5-CT/TOPO® TA Expression Kits. Each kit contains the pEXP5-NT/TOPO® or pEXP5-CT/TOPO® vector, other reagents to facilitate TOPO® Cloning, and One Shot® TOP10 Chemically Competent E. coli. Store the vectors at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.
