Siglec-2 / CD22蛋白產(chǎn)品信息

別名: CD22
表達(dá)方式: 
A DNA sequence encoding the mouse CD22 (BAE33829.1)(Met1-Arg708) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Mouse
表達(dá)宿主: Human Cells

Siglec-2 / CD22 Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Arg 19
分子量: 
The recombinant mouse CD22/Fc is a disulfide-linked homodimer. The reduced monomer comprises 931 amino acids and has a predicted molecular mass of 104.3 kDa. The apparent molecular mass of the protein is approximately 125 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

Siglec-2 / CD22 Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Siglec-2 / CD22蛋白背景綜述

CD22 is a molecule belonging to the immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family. It is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. The CD22 cell-surface adhesion molecule is capable of modulating B lymphocyte antigen receptor (BCR)-mediated signals, as well as the generation of BCR-independent signals. CD22 is an inhibitory coreceptor of the B-cell receptor (BCR), and plays a critical role in establishing signalling thresholds for B-cell activation. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. Interaction of CD22 to ligands in trans can regulate both B-cell migration as well as the BCR signaling threshold. However, a more complex role for CD22 has recently emerged, including a central role in a novel regulatory loop controlling the CD19/CD21-Src-family protein tyrosine kinase (PTK) amplification pathway that regulates basal signaling thresholds and intensifies Src-family kinase activation after BCR ligation. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1. Thus, the CD19/CD22 loop may be a potential therapeutic target in autoimmune disease for modulating B cell signaling.

參考文獻(xiàn)

Sato S. et al., 1998, Semin Immunol. 10 (4): 287-97.
Walker JA. et al., 2008, Immunology. 123 (3): 314-25.
Nitschke L. 2009, Immunol Rev. 230 (1): 128-43.
Tedder TF. et al., 2005, Adv Immunol. 88: 1-50.
Fujimoto M. et al., 2007, J Dermatol Sci. 46 (1): 1-9.

規(guī)格：50ug

關(guān) 鍵 詞：Mouse Siglec-2 / CD22 Protein,技術(shù)資料,廠家現(xiàn)貨,價格，實驗室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實驗儀器，廠家

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