H7N9 (A/Anhui/1/2013) 神經(jīng)氨酸酶(NA / Neuraminidase) 蛋白 Product Information

別名: NA
表達(dá)方式: 
A DNA sequence encoding the Influenza A virus (A/Anhui/1/2013(H7N9)) (AGI60300.1) neuraminidase (His36-Leu465) was expressed with an N-terminal polyhistidine tag.
種屬: Influenza A virus
表達(dá)宿主: Human Cells

H7N9 (A/Anhui/1/2013) 神經(jīng)氨酸酶(NA / Neuraminidase) 蛋白 QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
生物活性:
Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid.
The specific activity is >500 pmole/min/μg.
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測(cè)N端: His
分子量: 
The recombinant neuraminidase of Influenza A virus (A/Anhui/1/2013(H7N9)) comprises 448 amino acids and has a predicted molecular mass of 50.6 kDa. The apparent molecular mass of the protein is approximately 61-69 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

H7N9 (A/Anhui/1/2013) 神經(jīng)氨酸酶(NA / Neuraminidase)氨基酸序列

HHHHHHHHHH LEVLFQGPHL KPGCNCSHSQ PETTNTSQTI INNYYNETNI TNIQMEERTS RNFNNLTKGL CTINSWHIYG KDNAVRIGES SDVLVTREPY VSCDPDECRF YALSQGTTIR GKHSNGTIHD RSQYRALISW PLSSPPTVYN SRVECIGWSS TSCHDGKSRM SICISGPNNN ASAVVWYNRR PVAEINTWAR NILRTQESEC VCHNGVCPVV FTDGSATGPA DTRIYYFKEG KILKWESLTG TAKHIEECSC YGERTGITCT CRDNWQGSNR PVIQIDPVAM THTSQYICSP VLTDNPRPND PNIGKCNDPY PGNNNNGVKG FSYLDGANTW LGRTISTASR SGYEMLKVPN ALTDDRSKPI QGQTIVLNAD WSGYSGSFMD YWAEGDCYRA CFYVELIRGR PKEDKVWWTS NSIVSMCSST EFLGQWNWPD GAKIEYFL

H7N9 (A/Anhui/1/2013) 神經(jīng)氨酸酶(NA / Neuraminidase) 蛋白 Usage Guide

儲(chǔ)存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H7N9 (A/Anhui/1/2013) 神經(jīng)氨酸酶(NA / Neuraminidase) 蛋白描述

H7N9 is a subtype of Influenza virus A. On April 1, 2013, the World Health Organization (WHO) first reported 3 human infections with a new influenza A (H7N9) virus in China. Since then, additional cases have been reported. This new H7N9 virus is an avian (bird) influenza (flu) virus. Influenza (flu) is a respiratory infection in mammals and birds. The virus is divided into three main types (Influenza A, Influenza B, and Influenza C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA)). The influenza viral neuraminidase (NA) protein is a tetramer with an enzyme active site on the head of each monomer. Subtypes are further divided into strains; each genetically distinct virus isolate is usually considered to be a separate strain. NA protein is a second major surface antigen of the virion. Neuraminidase (NA) cleaves terminal sialic acid from glycoproteins or glycolipids. Thus, it functions to free virus particles from host cell receptors, to permit progeny virions to escape from the cell in which they arose, and so facilitate virus spread.

參考文獻(xiàn)

Suzuki T. et al., 2005, J Virol. 79: 11705-15.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.
Christophe F. et al., 2009, Science. 324: 1557-61.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.
Christophe F. et al., 2009, Science. 324:1557-61.

規(guī)格：50ug

關(guān) 鍵 詞：Influenza A H7N9 (A/Anhui/1/2013) NA / Neuraminidase,使用說(shuō)明,現(xiàn)貨，實(shí)驗(yàn)室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實(shí)驗(yàn)儀器，廠家

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