IL4R / Il4ra蛋白產(chǎn)品信息
別名: IL4R, Il4ra
表達方式:
A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Rat
表達宿主: Human Cells
IL4R / Il4ra Protein QC Testing
純度: > 90 % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Ile 26
分子量:
The recombinant rat IL4R/Fc is a disulfide-linked homodimer. The reduced monomer comprises 448 amino acids and has a predicted molecular mass of 50.9 kDa. The apparent molecular mass of the protein is approximately 66 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
IL4R / Il4ra Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL4R / Il4ra蛋白背景綜述
IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). In non-hematopoietic cells, the IL4Rα subunit associates with the IL13Rα chain and also serves as a functional complex for IL-13. IL4Rα is a single-pass type I transmembrane protein containing ser/Pro-rich regions in cytoplasmic domain similar to those present in the IL-2 receptor and GM-CSF receptor ß-chains. As a receptor for both IL4 and IL13, IL4Rα couples to the JAK1/2/3-STAT6 pathway, and is involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation, as well as promoting differentiation of Th2 cells. Two transcript variants encoding different isoforms of IL4Rα, a membrane-bound and a soluble form, have been found, and the soluble form of IL4Rα can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.
參考文獻
Grabstein K. et al.,1986, J Exp Med. 163: 1405-14.
Yokota T. et al., 1988, Immunol Rev. 102: 137-87.
Kotowicz K. et al., 1996, Int Immunol. 8: 1915-25.
Murata T. et al., 1998, Int J Mol Med. 1: 551-7.
Nelms K. et al., 1999, Ann Rev Immunol. 17: 701-38.
Hackstein H. et al.,1999, Tissue Antigens. 54 (5): 471-7.
Gessner A. et al., 2000, Immunobiology. 201 (3-4): 285-307.
規(guī)格:100ug
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