LIF蛋白產(chǎn)品信息

別名: LIF, HILDA
表達(dá)方式: 
A DNA sequence encoding the human LIF (P15018)(Met1-Phe202) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Human
表達(dá)宿主: Human Cells

LIF Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Ser 23
分子量: 
The recombinant human LIF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 421 amino acids and has a predicted molecular mass of 46.7 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

LIF Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LIF蛋白背景綜述

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It shows a wide range of biologic activities that include the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. LIF is considered as a direct transcriptional target of STAT5 in myeloid cell lines: STAT5 binds to LIF promoter, and LIF expression is increased after activation of the JAK2/STAT5 pathway. LIF expression is significantly increased in patients with chronic myeloproliferative neoplasms with and without activating mutations of the pathway, indicating that LIF might play an important role in STAT5-mediated oncogenesis.

參考文獻(xiàn)

Salas EM. et al. (2011) Genes Cancer. 2 (5): 593-6.
Chodorowska G. et al. (2004) Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93.
García-Campaa AM. et al. (2007) Electrophoresis. 28 (1-2): 208-32.

規(guī)格：20ug

關(guān) 鍵 詞：LIF Protein,生活蛋白質(zhì),技術(shù)資料,價(jià)格，實(shí)驗(yàn)室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實(shí)驗(yàn)儀器，廠家

CANSPEC品質(zhì)提供的LIF Protein|生活蛋白質(zhì)|技術(shù)資料|價(jià)格性能**，其優(yōu)良的品質(zhì)滿足您不同實(shí)驗(yàn)的多種需求。

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