NT5E / CD73蛋白產(chǎn)品信息

別名: NT5E
表達(dá)方式: 
A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.
種屬: Rat
表達(dá)宿主: Human Cells

NT5E / CD73 Protein QC Testing

純度: > 90 % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測(cè)N端: Trp 29
分子量: 
The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of  the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

NT5E / CD73 Protein Usage Guide

儲(chǔ)存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NT5E / CD73蛋白背景綜述

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

參考文獻(xiàn)

Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.

規(guī)格：10ug

關(guān) 鍵 詞：Rat NT5E / CD73 Protein,廠家現(xiàn)貨,價(jià)格，實(shí)驗(yàn)室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實(shí)驗(yàn)儀器，廠家

CANSPEC品質(zhì)提供的Rat NT5E / CD73 Protein|廠家現(xiàn)貨|價(jià)格性能**，其優(yōu)良的品質(zhì)滿(mǎn)足您不同實(shí)驗(yàn)的多種需求。

本產(chǎn)品由上海創(chuàng)賽品質(zhì)提供，歡迎咨詢(xún)國(guó)內(nèi)免費(fèi)服務(wù)專(zhuān)線(xiàn)：400-608-7598，更多產(chǎn)品信息請(qǐng)登錄www.ahbcpqn.cn 或 www.4006087598.com