IL-23 / IL23A & IL12B Heterodimer蛋白產(chǎn)品信息

別名: 
Interleukin-23 (IL23A & IL12B Heterodimer)
表達(dá)方式: 
A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.
種屬: Mouse
表達(dá)宿主: Human Cells

IL-23 / IL23A & IL12B Heterodimer Protein QC Testing

純度:  (33.1+31.5+32.9) % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測(cè)N端: Val 22 & Met 23
分子量: 
The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

IL-23 / IL23A & IL12B Heterodimer Protein Usage Guide

儲(chǔ)存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-23 / IL23A & IL12B Heterodimer蛋白背景綜述

Interleukin-23 subunit alpha, also known as IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19 and IL23A, is a secreted protein which belongs to the IL-6 superfamily. IL-23A associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Interleukin-12 subunit beta, also known as cytotoxic lymphocyte maturation factor 40 kDa subunit, NK cell stimulatory factor chain 2, NKSF2 and IL12B, is secreted protein which belongs to the type I cytokine receptor family and Type 3 subfamily. IL12B / NKSF2 is a ctokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. IL12B / NKSF2 associates with IL-23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues.

參考文獻(xiàn)

Altare F. et al., 1998, J Clin Invest. 102: 2035-40.
Oppmann B. et al., 2000, Immunity. 13: 715-25.
Picard C. et al., 2002, Am J Hum Genet. 70: 336-48.
Parham C. et al., 2002, J Immunol. 168: 5699-708.
Pirhonen J. et al., 2002, J Immunol. 169: 5673-8.

規(guī)格：10ug

關(guān) 鍵 詞：Mouse IL-23 / IL23A & IL12B Heterodimer Protein,價(jià)格,廠家現(xiàn)貨，實(shí)驗(yàn)室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實(shí)驗(yàn)儀器，廠家

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