CD22蛋白產(chǎn)品信息

別名: CD22
表達(dá)方式: 
A DNA sequence encoding the cynomolgus CD22 (Met1-Arg686) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Cynomolgus
表達(dá)宿主: Human Cells

CD22 Protein QC Testing

純度: > 90 % as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測(cè)N端: Ser 20
分子量: 
The recombinant cynomolgus CD22 is a disulfide-linked homodimer. The reduced monomer comprises 908 amino acids and has a calculated molecular mass of 102.1 KDa.The apparent molecular mass of the protein is approximately 117 KDa respectively in SDS-PAGE.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CD22 Protein Usage Guide

儲(chǔ)存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD22蛋白背景綜述

CD22 is a molecule belonging to the immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family. It is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. The CD22 cell-surface adhesion molecule is capable of modulating B lymphocyte antigen receptor (BCR)-mediated signals, as well as the generation of BCR-independent signals. CD22 is an inhibitory coreceptor of the B-cell receptor (BCR), and plays a critical role in establishing signalling thresholds for B-cell activation. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. Interaction of CD22 to ligands in trans can regulate both B-cell migration as well as the BCR signaling threshold. However, a more complex role for CD22 has recently emerged, including a central role in a novel regulatory loop controlling the CD19/CD21-Src-family protein tyrosine kinase (PTK) amplification pathway that regulates basal signaling thresholds and intensifies Src-family kinase activation after BCR ligation. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1. Thus, the CD19/CD22 loop may be a potential therapeutic target in autoimmune disease for modulating B cell signaling.

參考文獻(xiàn)

Sato S. et al., 1998, Semin Immunol. 10 (4): 287-97.
Walker JA. et al., 2008, Immunology. 123 (3): 314-25.
Nitschke L. 2009, Immunol Rev. 230 (1): 128-43.
Tedder TF. et al., 2005, Adv Immunol. 88: 1-50.
Fujimoto M. et al., 2007, J Dermatol Sci. 46 (1): 1-9.

規(guī)格：20ug

關(guān) 鍵 詞：Cynomolgus CD22 Protein,現(xiàn)貨,技術(shù)資料，實(shí)驗(yàn)室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實(shí)驗(yàn)儀器，廠家

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