FZD4 / Frizzled-4蛋白產(chǎn)品信息

別名: FZD4
表達方式: 
A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.
種屬: Rat
表達宿主: Human Cells

FZD4 / Frizzled-4 Protein QC Testing

純度: > (13.3+78.9+5.2 )% as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Phe 38
分子量: 
The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

FZD4 / Frizzled-4 Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FZD4 / Frizzled-4蛋白背景綜述

FZD4, also known as CD344, belongs to the the frizzle family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. FZD4 is the receptor for Wnt proteins.  In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin, promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. FZD4 plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin.

參考文獻

Omoto S. et al., 2004, Ophthalmic Genet. 25 (2): 81-90.
Toomes C. et al., 2004, Invest Ophthalmol Vis Sci. 45 (7): 2083-90.
Sagara N. et al., 2001, Biochem Biophys Res. Commun. 282 (3): 750-6.

規(guī)格：50ug

關(guān) 鍵 詞：Rat FZD4 / Frizzled-4 Protein,價格,技術(shù)資料，實驗室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實驗儀器，廠家

CANSPEC品質(zhì)提供的Rat FZD4 / Frizzled-4 Protein|價格|技術(shù)資料性能**，其優(yōu)良的品質(zhì)滿足您不同實驗的多種需求。

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