CALR / Calreticulin蛋白產品信息

別名: CALR, CRTC
表達方式: 
A DNA sequence encoding the human CALR (P27797) (Met1-Ala413) was expressed with a polyhistidine tag at the C-terminus.
種屬: Human
表達宿主: Human Cells

CALR / Calreticulin Protein QC Testing

純度: > 95% as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Glu 18
分子量: 
The recombinant human CALR consists of  407 amino acids and predicts a molecular mass of 47.4 KDa. It migrates as an approximately 58 KDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

CALR / Calreticulin Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CALR / Calreticulin蛋白背景綜述

As a multifunctional protein, calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. It is a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients that contain anti-Ro/SSA antibodies. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors.

參考文獻

Michalak M. et al., 2002, Biochim Biophys Acta. 1600 (1-2): 32-7
Chao MP. et al., 2010, Sci Transl Med. 2 (63): 63-94.
Andrin C. et al., 1998, Biochemistry. 37 (29): 10386-94.

規(guī)格：20ug

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