KIT / c-KIT蛋白產品信息

別名: KIT
表達方式: 
A DNA sequence encoding the rat KIT (Q63116)(Met1-Thr522) was expressed, fused with a polyhistidine tag at the C-terminus.
種屬: Rat
表達宿主: Human Cells

KIT / c-KIT Protein QC Testing

純度: > 95 % as determined by SDS-PAGE SDS-PAGE
內**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預測N端: Ser 25
分子量: 
The recombinant rat KIT comprises 509 amino acids and predicts a molecular mass of 56.9 kDa. The apparent molecular mass of  the recombinant protein is approximately 83 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.

KIT / c-KIT Protein Usage Guide

儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KIT / c-KIT蛋白背景綜述

Mast / stem cell growth factor receptor, also known as Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117 and KIT, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. CD117 / KIT contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 / KIT is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). CD117 is the receptor for the cytokine stem cell factor (SCF), when this receptor binds to SCF it forms a dimer that activates signaling through second messengers. Signaling through CD117 plays a role in cell survival, proliferation, and differentiation. Defects in CD117 / KIT are a cause of piebaldism which is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Defects in CD117 / KIT have also been associated with testicular tumors (TEST) which is a common solid malignancy in males.

參考文獻

Fleischman RA. et al., 1992, J Clin Invest. 89:1713-7.
Spritz RA. et al., 1992, Am J Hum Genet. 50:261-9.
Syrris P. et al., 2000, Am J Med Genet. 95:79-81.
Leong KG. et al., 2008, Nature. 456 (7223): 804.

規(guī)格：20ug

關 鍵 詞：Rat KIT / c-KIT Protein,參數,產品詳情，實驗室耗材，生化試劑，上海創(chuàng)賽，博飛美科，實驗儀器，廠家

CANSPEC品質提供的Rat KIT / c-KIT Protein|廠家|參數|產品詳情性能**，其優(yōu)良的品質滿足您不同實驗的多種需求。

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