AMY2A / Alpha-amylase蛋白產(chǎn)品信息
別名: AMY2A
表達方式:
A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.
種屬: Human
表達宿主: Human Cells
AMY2A / Alpha-amylase Protein QC Testing
純度: > 85% as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Gln 16
分子量:
The recombinant human AMY2A consists of 507 amino acids and predicts a molecular mass of 57.3 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
AMY2A / Alpha-amylase Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
AMY2A / Alpha-amylase蛋白背景綜述
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase is the major form of amylase found in humans and other mammals. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human α-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.
參考文獻
Abe A. et al., 2005, FEBS J. 272 (23): 6145-53.
Aghajari N. et al., 1998, Protein Sci. 7 (3): 564-72.
Ramasubbu N. et al., 1996, Acta Crystallographica Section D Biological Crystallography. 52 (3): 435-46.
規(guī)格:50ug
關(guān) 鍵 詞:AMY2A / Alpha-amylase Protein,技術(shù)資料,產(chǎn)品詳情 ,實驗室耗材,生化試劑,上海創(chuàng)賽,博飛美科,實驗儀器,廠家
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