RPE / RPE2-1蛋白產(chǎn)品信息
別名: RPE
表達(dá)方式:
A DNA sequence encoding the mouse RPE (Q8VEE0) (Met1-Arg228) was expressed with a C-terminal polyhistidine tag.
種屬: Mouse
表達(dá)宿主: Human Cells
RPE / RPE2-1 Protein QC Testing
純度: >90% as determined by SDS-PAGE SDS-PAGE
內(nèi)**: < 1.0 EU per μg of the protein as determined by the LAL method
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Met
分子量:
The recombinant mouse RPE comprises 239 amino acids and has a predicted molecular mass of 26.4 kDa. The apparent molecular mass of the protein is approximately 27 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩沖液: Lyophilized from sterile PBS, pH7.4.
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
RPE / RPE2-1 Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
RPE / RPE2-1蛋白背景綜述
The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE), orotidine 5'-monophosphate decarboxylase (OMPDC), and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation, or origin, site, at which DNA synthesis begins. In enzymology, a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence, RPE has one substrate, L-ribulose 5-phosphate, and one product, L-xylulose 5-phosphate. RPE belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase, UlaE, and SgaU.
參考文獻(xiàn)
Yew WS. et al., 2002, J Bacteriol. 184 (1): 302-6.
Shimaoka M. et al., 2005, Biosci Biotechnol Biochem. 69 (7): 1248-55.
Salinero KK. et al., 2009, BMC Genomics. 10: 351.
規(guī)格:50ug
關(guān) 鍵 詞:Mouse RPE / RPE2-1 Protein,產(chǎn)品詳情,實(shí)驗室耗材,生化試劑,上海創(chuàng)賽,博飛美科,實(shí)驗儀器,廠家
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