EGF蛋白產(chǎn)品信息
產(chǎn)品名稱：重組人 EGF / Epidermal Growth Factor 蛋白|EGF Protein|技術(shù)資料|參數(shù)
別名: EGF,   HOMG4,   URG
表達(dá)方式: A DNA sequence encoding the mature form of human EGF ( NP_001954.2 ) ( Asn 971 - Arg 1023 ) was expressed and purified, with an initial Met at the N-terminus
種屬: Human
表達(dá)宿主: E. coli

EGF蛋白質(zhì)控指標(biāo)
純度: > 92 % as determined by SDS-PAGE SDS-PAGE生物活性:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
( Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415. )
The ED50 for this effect is typically 20 - 100 pg/mL
內(nèi)**: Please contact us for more information.
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測(cè)N端: Met 1
分子量: The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 KDa as estimated in SDS-PAGE under reducing conditions.
緩沖液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
 
EGF蛋白使用指南
儲(chǔ)存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGF蛋白背景綜述
Human epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serves as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved 6 cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing 9 EGF units, and is proteolytically cleaved to generate the 53-amino acid peptide hormone.
參考文獻(xiàn)
Carpenter, G and Cohen, S. 1990. J. Biol. Chem.265: 7709-7712.
Hommel, U. et al., 1992, J. Mol. Biol. 227: 271-282.
Goodsell, D.S. et al., 2003, Stem Cells. 21: 702-703.

包裝：50ug  200ug

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