EGF蛋白產(chǎn)品信息
產(chǎn)品名稱：重組人 EGF / Epidermal Growth Factor 蛋白|EGF蛋白|技術(shù)資料|現(xiàn)貨
EGF 別名: HOMG4, URG,  EGF
表達(dá)方式: A DNA sequence encoding the mature soluble form of human EGF (NP_001954.2) (Asn 971- Arg 1023) was fused with the Fc region of human IgG1 at the N-terminus.
種屬: Human
表達(dá)宿主: Human Cells

EGF Protein QC Testing
純度: >90 % as determined by SDS-PAGE. SDS-PAGE:
生物活性:Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.1-0.6 ng/mL.
內(nèi)**: < 1.0 EU per 1μg of the protein as determined by the LAL method.
穩(wěn)定性: Samples are stable for up to twelve months from date of receipt at -70℃
預(yù)測N端: Glu 20
分子量: The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.
緩沖液: Lyophilized from sterile PBS , pH 7.4
Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Please contact us for any concerns or special requirements.
EGF Protein Usage Guide
儲存方法: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted into smaller quantities for optimal storage and usage; Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EGF蛋白背景綜述
Human epidermal growth factor (EGF), also known as urogastrone (URG) since it was isolated from urine based on its inhibitory effect on gastric secretion, is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. EGF is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble EGF is a 6 kDa peptide corresponding to the EGF unit located proximal to the transmembrane domain. EGF is expressed in kidney, salivary gland, cerebrum and prostate, and plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. As a potent mitogen, EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro. Furthermore, EGF is also identified as a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4), a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion.
參考文獻(xiàn)
Ciardiello, F. et al., 1991, Proc. Natl. Acad. Sci. USA. 88: 7792-7796.
Gout, I. et al., 1992, Biochem. J. 288: 395-405.
Harris, R.C. et al., 2003, Exp. Cell. Res. 284: 2-13.
Dreux, A.C. et al., 2006, Atherosclerosis. 186: 38-53.
Groenestege, W.M.T. et al., 2007, J. Clin. Invest. 117: 2260-2267.

包裝：200ug

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