產(chǎn)品名稱:Mouse IFN-γ ELISPOT Pair

Mouse IFN-γ ELISPOT Pair 產(chǎn)品介紹：

Material Number: 551881
Size: 5 plates
Component: 51-2525KZ
Description: Mouse IFN-γ ELISPOT Capture Antibody
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative,
0.2μm filtered. Endotoxin level is ≤0.01 ng/μg of protein.
Component: 51-1818KA
Description: Mouse IFN-γ ELISPOT Detection Antibody
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium
azide.
Mouse IFN-γ ELISPOT Pair Description：

The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular
protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture
and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay
has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of
the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay
include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed
biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation
assays, flow cytometry, or other methods of analysis.

Mouse IFN-γ ELISPOT Pair Preparation and Storage：

Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Mouse IFN-γ ELISPOT Pair Suggested Companion Products：

Catalog Number Name Size Clone
551951 AEC Substrate Set 10 plates (none)
557630 HRP Streptavidin for ELISPOT 1.0 ml (none)