產(chǎn)品名稱:APC 倉鼠抗小鼠 CD3e 抗體；APC Hamster Anti-Mouse CD3e

中文名稱:APC 倉鼠抗小鼠 CD3e 抗體

英文名稱:APC Hamster Anti-Mouse CD3e

APC Hamster Anti-Mouse CD3e 產(chǎn)品介紹：

Material Number: 553066
Alternate Name: CD3ε chain
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: 145-2C11
Immunogen: H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Isotype: Armenian Hamster IgG1, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

The 145-2C11 antibody reacts with the 25-kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, 
mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several 
cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed 
(memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can 
activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes.

APC Hamster Anti-Mouse CD3e Preparation and Storage：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed