產(chǎn)品名稱:FITC 大鼠抗小鼠 CD25 抗體；FITC Rat Anti-Mouse CD25

中文名稱:FITC 大鼠抗小鼠 CD25 抗體

英文名稱:FITC Rat Anti-Mouse CD25

FITC Rat Anti-Mouse CD25 產(chǎn)品介紹：

Material Number: 553072
Alternate Name: IL-2 Receptor α chain, p55
Size: 0.5 mg
Concentration: 0.5 mg/ml
Clone: 7D4
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Isotype: Rat (LEW) IgM, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.



FITC Rat Anti-Mouse CD25 Description：

The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all 
mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) 
chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not 
express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low 
TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- 
Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of 
T-cell development in fetal and ***** thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the 
maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 
3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks 
binding of IL-2 to CD25.

FITC Rat Anti-Mouse CD25 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.