產(chǎn)品名稱:FITC 大鼠抗小鼠 CD45R/B220 抗體；FITC Rat Anti-Mouse CD45R/B220

中文名稱:FITC 大鼠抗小鼠 CD45R/B220 抗體

英文名稱:FITC Rat Anti-Mouse CD45R/B220

FITC Rat Anti-Mouse CD45R/B220 產(chǎn)品介紹：

Material Number: 553087
Size: 0.1 mg
Concentration: 0.5 mg/ml
Clone: RA3-6B2
Immunogen: Mouse Pre-B Tumor
Isotype: Rat IgG2a, κ
Reactivity:QC Testing: Mouse
Reported: Human
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide



FITC Rat Anti-Mouse CD45R/B220 Description：

The RA3-6B2 antibody reacts with an epitope on the extracellular domain of CD45 glycoprotein which is dependent upon the expression of 
exon A and specific carbohydrate residues. It is expressed on B lyphocytes at all stages from pro-B through mature and activated B cell, but it 
is decreased on plasma cells and a subset of memory B cells. Levels of expression of CD45R/B220 on the B-cell lineage appear to be 
developmentally regulated. It is also found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on 
lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice 
injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and 
macrophage progenitors in fetal liver. The CD45R/B220 antigen is not on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted 
CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP 
catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, 
respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation 
state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R/B220 is commonly 
used as a pan B-cell marker; however, CD19 expression, detected by mAb 1D3, is reported to be more restricted to the B-cell lineage. mAb 
RA3-6B2 has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. 
Cross-reaction of RA3-6B2 mAb with activated human T lymphocytes has been observed. 

FITC Rat Anti-Mouse CD45R/B220 Preparation and Storage：

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.