產(chǎn)品名稱:PE 大鼠抗小鼠 CD45R/B220 抗體；PE Rat Anti-Mouse CD45R/B220
中文名稱:PE 大鼠抗小鼠 CD45R/B220 抗體
英文名稱:PE Rat Anti-Mouse CD45R/B220
PE Rat Anti-Mouse CD45R/B220 產(chǎn)品介紹：
Material Number: 553089
Size: 0.1 mg
Concentration: 0.2 mg/ml
Clone: RA3-6B2
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Isotype: Rat IgG2a, κ
Reactivity: QC Testing: Mouse
Reported: Human
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
PE Rat Anti-Mouse CD45R/B220 Description：
The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the
transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B
lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells.
The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T
cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK
cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell
precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been
reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine
Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly
variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The
CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell
and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker; however, CD19 expression, detectable by
the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody
(clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses.
Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.
PE Rat Anti-Mouse CD45R/B220 Preparation and Storage：
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
