產(chǎn)品名稱:PE 大鼠抗小鼠 Ly-6A/E 抗體;PE Rat Anti-Mouse Ly-6A/E
中文名稱:PE 大鼠抗小鼠 Ly-6A/E 抗體
英文名稱:PE Rat Anti-Mouse Ly-6A/E
PE Rat Anti-Mouse Ly-6A/E 產(chǎn)品介紹:
Material Number: 553108
Alternate Name: Sca-1
Size: 0.2 mg
Concentration: 0.2 mg/ml
Clone: D7
Immunogen: IL-2-dependent mouse T-cell line CTL-L
Isotype: Rat (LEW) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
PE Rat Anti-Mouse Ly-6A/E Description:
The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a
phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of
mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR,
129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T
lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral
lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies
with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to
be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of
FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated
E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No.
559971) to identify HSC.
PE Rat Anti-Mouse Ly-6A/E Preparation and Storage:
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed by gel
filtration chromatography.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.