產品名稱:FITC 大鼠抗小鼠 Ly-6A/E 抗體；FITC Rat Anti-Mouse Ly-6A/E
中文名稱:FITC 大鼠抗小鼠 Ly-6A/E 抗體
英文名稱:FITC Rat Anti-Mouse Ly-6A/E
FITC Rat Anti-Mouse Ly-6A/E 產品介紹：
Material Number: 553335
Alternate Name: Sca-1
Size: 0.5 mg
Concentration: 0.5 mg/ml
Clone: E13-161.7
Immunogen: BALB/c mouse-derived "pre-T" cell hybridoma
Isotype: Rat (WI) IgG2a, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
FITC Rat Anti-Mouse Ly-6A/E Description：
The E13-161.7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca1 (Ly-6A/E), a
phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cell (HSC) in mice with both Ly-6
haplotypes. Sca-1+ HSC are found in the ***** bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic
hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the *****. In mice expressing the Ly-6.2 haplotype (e.g., AKR,
C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and
peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population,
and in several non-hematopoietic tissues. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few
Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the
Sca-1 antigen. Studies with the D7 antibody (Cat. No. 557403) have demonstrated that Ly-6A/E may be involved in the regulation of B and T
lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb can block binding
of FITC-conjugated D7 antibody (anti-Ly-6A/E, Cat. No. 557405) to mouse splenocytes, but purified mAb D7 (Cat. No. 557403) is unable to
block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the
Mouse Lineage Panel (Cat. No. 559971) to identify HSC
FITC Rat Anti-Mouse Ly-6A/E Preparation and Storage：
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
