產(chǎn)品名稱:PE 大鼠抗小鼠 CD45RA 抗體；PE Rat Anti-Mouse CD45RA
中文名稱:PE 大鼠抗小鼠 CD45RA 抗體
英文名稱:PE Rat Anti-Mouse CD45RA
PE Rat Anti-Mouse CD45RA 產(chǎn)品介紹：
Material Number: 553380
Size: 0.2 mg
Concentration: 0.2 mg/ml
Clone: 14.8
Immunogen: Radiation-induced NZC mouse B lymphoma WEHI-279
Isotype: Rat IgG2b, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
PE Rat Anti-Mouse CD45RA Description：
The 14.8 clone has been reported to react with an exon A-dependent epitope of the CD45 protein, which is found at high density on B cells
and at low density on peripheral T cytotoxic/suppressor cells and a very small subset of thymocytes. Nearly all B-lineage cells, including
B-cell precursors in fetal liver and ***** bone marrow and Ig-secreting cells, but not hematopoietic stem cells or myeloid progenitors, have
been reported to be detectable by mAb 14.8. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular
(COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of
exons 4, 5, and 6 (designated A, B, and C, respectively), plus, differing levels of glycosylation. The CD45 isoforms detected in the mouse are
cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal
transduction. mAb 14.8 has been reported to enhance the proliferative effect of PHA on purified spleen T cells, possibly by replacing a signal
normally delivered by accessory cells, to enhance isotype switching during in vitro B-cell responses, and to inhibit antigen-induced p21 [ras]
activation.
PE Rat Anti-Mouse CD45RA Preparation and Storage：
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
