產(chǎn)品名稱:FITC 大鼠抗小鼠 CD49b 抗體；FITC Rat Anti-Mouse CD49b
中文名稱:FITC 大鼠抗小鼠 CD49b 抗體
英文名稱:FITC Rat Anti-Mouse CD49b
FITC Rat Anti-Mouse CD49b 產(chǎn)品介紹：
Material Number: 553857
Alternate Name: itga2; Integrin alpha-2; DX5; Pan NK cell marker; VLAA2; VLA-2 alpha chain
Size: 0.5 mg
Concentration: 0.5 mg/ml
Clone: DX5
Immunogen: Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2
Isotype: Rat (LEW) IgM, κ
Reactivity: QC Testing: Mouse
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
FITC Rat Anti-Mouse CD49b Description：
The rat anti-mouse CD49b monoclonal antibody (clone DX5) specifically binds to the integrin α2 chain (CD49b). CD49b is a 150 kDa
transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1) to form the integrin α2β1 complex known as VLA-2. The
rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most
mouse strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not
NOD). The DX5 antibody also recognizes platelets that express high levels of CD49b. Multiparameter flow cytometric analysis has
demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody
(clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ cell subsets are found, especially among the
CD3-positive cell population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody
(clone DX5) when cultured in the presence of recombinant human IL-2. The resulting DX5-negative cells have weakened cytotoxic activity
when compared to the remaining DX5+ cells. This indicates that the DX5 antibody distinguishes functional subsets of NK cells. No activation
or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed. Staining of splenic NK cells with this antibody reportedly
can be blocked by hamster anti-mouse CD49b antibody (clone HMα2).
FITC Rat Anti-Mouse CD49b Preparation and Storage：
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
