產(chǎn)品名稱:PE 小鼠抗人 CD8 抗體；PE Mouse Anti-Human CD8

中文名稱:PE 小鼠抗人 CD8 抗體

英文名稱:PE Mouse Anti-Human CD8

PE Mouse Anti-Human CD8 產(chǎn)品介紹：

Material Number: 555367
Alternate Name: CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
Size: 100 Tests
Vol. per Test: 20 μl
Clone: RPA-T8
Isotype: Mouse IgG1, κ
Reactivity: QC Testing: Human
Tested in Development: Rhesus, Cynomolgus, Baboon
Workshop: IV T171; V T-CD08.03; VI 6T-CD8.1, 6T-081
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% 
sodium azide.

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of 
the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by 
some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when 
disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas 
some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular 
IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a 
co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a 
Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not 
cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of 
baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are 
observed in non-human primates compared to normal human donors.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.