產(chǎn)品名稱:APC 小鼠抗人 CD8 抗體；APC Mouse Anti-Human CD8

中文名稱:APC 小鼠抗人 CD8 抗體

英文名稱:APC Mouse Anti-Human CD8

APC Mouse Anti-Human CD8 產(chǎn)品介紹：

Material Number: 555369
Alternate Name: CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
Size: 100 Tests
Vol. per Test: 20 μl
Clone: RPA-T8
Isotype: Mouse IgG1, κ
Reactivity: QC Testing: Human
Tested in Development: Rhesus, Cynomolgus, Baboon
Workshop: IV T171; V T-CD08.03; VI 6T-CD8.1, 6T-081
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α). CD8α is a type I transmembrane glycoprotein and a member of 
the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by 
some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when 
disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas 
some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular 
IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a 
co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a 
Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not 
cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of 
baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are 
observed in non-human primates compared to normal human donors.

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.