產(chǎn)品名稱:PerCP-Cy?5.5 Mouse anti-ERK1/2 (pT202/pY204)
PerCP-Cy?5.5 Mouse anti-ERK1/2 (pT202/pY204) 產(chǎn)品介紹：
Material Number: 560115
Alternate Name: p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Size: 50 tests
Vol. per Test: 20 μl
Clone: 20A
Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide
Isotype: Mouse IgG1
Reactivity: Confirmed by flow cytometry: Human
Confirmed by western blot using purified antibody (Cat. No. 612358 or
612359): Human, Mouse, Rat
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
PerCP-Cy?5.5 Mouse anti-ERK1/2 (pT202/pY204) Description：
The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events
at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the
proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases,
ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as
growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and
eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation
of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other
cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the
active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the
cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers.
PerCP-Cy?5.5 Mouse anti-ERK1/2 (pT202/pY204) Preparation and Storage：
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
PerCP-Cy?5.5 Mouse anti-ERK1/2 (pT202/pY204) Suggested Companion Products：
Catalog Number Name Size Clone
554655 Fixation Buffer 100 ml (none)
557870 Fix Buffer I 250 ml (none)
558050 Perm Buffer III 125 ml (none)
558052 Perm Buffer II 125 ml (none)
557885 Perm/Wash Buffer I 125 ml (none)
558049 Lyse/Fix Buffer 5X 250 ml (none)
