產(chǎn)品名稱 :Abcam Cell Cycle In-Cell ELISA試劑盒(Fluorescent) (ab140363)

Abcam Cell Cycle In-Cell ELISA試劑盒(Fluorescent) (ab140363)產(chǎn)品概述：

ab140363 is an In-Cell ELISA (ICE) assay kit that uses quantitative immunocytochemistry to measure levels of Cdk2 protein phosphorylated Tyr15 and Histone H3 protein phosphorylated Ser10 levels in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized antibodies. Relative target levels are quantified using secondary antibodies conjugated to either horseradish peroxidase (HRP) or alkaline phosphatase (AP) which generate signal through the use of two spectrally distinct fluorogenic substrates. Fluorescence is measured using a standard fluorescent spectrophotometer and relative levels of target proteins are quantified. Optionally, antibody signal intensity can be normalized to the total cell amount using Janus Green stain. In-Cell ELISA (ICE) technique generates quantitative data with specificity similar to Western blotting, but with much greater quantitative precision and higher throughput due to the greater dynamic range and linearity of fluorescence detection and the ability to run up to 96 samples in parallel. This method rapidly fixes the cells in situ, stabilizing the in vivo levels of proteins and their post-translational modifications, and thus essentially eliminates changes during sample handling, such as preparation of protein extracts.

Abcam Cell Cycle In-Cell ELISA試劑盒(Fluorescent) (ab140363)種屬反應(yīng)性：

與反應(yīng): Mouse, Human

Abcam Cell Cycle In-Cell ELISA試劑盒(Fluorescent) (ab140363)組件：

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關(guān)鍵字： Cell Cycle In-Cell ELISAkit (Fluorescent) ，ab140363，Abcam，試劑盒，優(yōu)惠促銷，現(xiàn)貨促銷，上海創(chuàng)賽科技代理，創(chuàng)賽中國(guó)

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