產(chǎn)品名稱 :Abcam 人Axl ELISA試劑盒SimpleStep |(ab208987)
Abcam 人Axl ELISA試劑盒SimpleStep |(ab208987) 產(chǎn)品概述:
Abcam’s Axl in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Axl protein in human serum, plasma, cell culture supernatant, urine, saliva and milk.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Abcam 人Axl ELISA試劑盒SimpleStep |(ab208987) 存放說明:
Store at +4°C. Please refer to protocols
Abcam 人Axl ELISA試劑盒SimpleStep |(ab208987) 種屬反應(yīng)性:
與反應(yīng): Human
不與反應(yīng): Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
Abcam 人Axl ELISA試劑盒SimpleStep |(ab208987) 組件:
組件
|
1 x 96 tests
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10X Human Axl Capture Antibody
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1 x 600μl
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10X Human Axl Detector Antibody
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1 x 600μl
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10X Wash Buffer PT (ab206977)
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1 x 20ml
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Antibody Diluent CPI
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1 x 6ml
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Human Axl Lyophilized Recombinant Protein
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2 vials
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Plate Seals
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1 unit
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Sample Diluent NS
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1 x 50ml
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SimpleStep Pre-Coated 96-Well Microplate
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1 unit
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Stop Solution
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1 x 12ml
|
TMB Substrate
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1 x 12ml
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關(guān)鍵字:人Axl ELISAkit SimpleStep| ab208987|Abcam|現(xiàn)貨促銷|上海創(chuàng)賽科技代理|創(chuàng)賽中國|上海創(chuàng)賽
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