Synonyms :

Transforming Growth Factor-α, Sacroma growth factor, TGF-type I, ETGF

Description :

TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-α are cross-species reactive. Recombinant human TGF-α is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Catalog #:

100-16A

Source :

E.coli

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted TGF-α is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per ug (1EU/µg).

Biological Activity :

The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.

Selected References :

Selected List of References Citing This PeproTech Product

AA Sequence :

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA

PubMed Link :

TGFalpha

Country of Origin :

USA