Synonyms :

Transforming Growth Factor-beta2, Glioblastoma-derived T cell suppressor factor, BSC-1, Cetermin, Polyergin

Description :

The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-β2 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Catalog #:

100-35

Source :

(BTI-Tn-5B1-4) High-5 Insect Cells*/i>

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted TGF-β2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED₅₀ was found to be ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.

Selected References :

Selected List of References Citing This PeproTech Product

AA Sequence :

ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS

PubMed Link :

TGF+AND+beta+AND+2

Country of Origin :

USA