Synonyms :

Soluble Frizzled Related Protein-1, Secreted Apoptosis Related Protein-2, SARP-2

Description :

Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Recombinant human sFRP-1 is a glycosylated 283 amino acid protein containing a cysteine rich Frizzled homologous domain.

Catalog #:

120-29

Source :

HeLa cells

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted sFRP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 97% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED₅₀ is 0.3–0.5 μg/ml.

AA Sequence :

SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK

PubMed Link :

sFRP-1

Country of Origin :

USA