Synonyms :

Tumor Necrosis Factor-beta, TNFSF1, Lymphotoxin-alpha (LT-alpha)

Description :

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes incuding cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Recombinant human TNF-β is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide linked homotrimer structure in solution. Due to glycosylation, TNF-β has an approximate MW of 19.3 kDa based on SDS-PAGE gel amd Mass Spectrometry.* Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Catalog #:

300-01BH

Source :

(BTI-Tn-5B1-4) High-5 Insect Cells*

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted TNF-β is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

The ED₅₀ was determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

AA Sequence :

GVGLTPSAAQ TARQHPKMHL AHSTLKPAAH LIGDPSKQNS LLWRANTDRA FLQDGFSLSN NSLLVPTSGI YFVYSQVVFS GKAYSPKATS SPLYLAHEVQ LFSSQYPFHV PLLSSQKMVY PGLQEPWLHS MYHGAAFQLT QGDQLSTHTD GIPHLVLSPS TVFFGAFAL

PubMed Link :

TNFbeta+AND+human

Country of Origin :

USA