Synonyms :

Soluble Tumor Necrosis Factor type II, TNFRSF1B, TNFR75, p75, CD120b, TNFR80, p80

Description :

TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-α but manifests a lower affinity to TNF-β. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-α induced activities by acting as a decoy receptor. Recombinant human sTNFRII is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFRII protein.

Catalog #:

310-12

Source :

E.coli

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted sTNFRII is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Biological Activity :

Determined by its inhibitory effect of the TNF-α mediated cytotoxicity in murine L-929 cells. The ED₅₀ for this effect in the presence of 0.25 ng/ml of recombinant human TNF-α, is 0.125 µg/ml.

AA Sequence :

MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP

PubMed Link :

TNF+AND+Receptor-2

Country of Origin :

USA