Catalog Number:  C22-101-10
Source:  Escherichia coli.
Molecular Weight:  Approximately 18.6kDa, a single non-glycosylated polypeptide chain containing 161 amino acids.
Quantity:  2μg/10μg/100μg/1000μg
AA Sequence:  MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLKESLLEDFKGY
 LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK
 AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay using murine MC/9-2 cells is less than 2 ng/ml, corresponding to a specific activity of＞ 5.0 × 105 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rHuIL-10 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Human Interleukin-10
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α
-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated
hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is
active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain
disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Rα
/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving
JAK1, TYK2, and STAT3 . IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical
molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2
responses but suppresses antigen presentation and Th1 proinflammatory responses.