Catalog Number:  C22-121-02
Source:  Escherichia coli.
Molecular Weight:  Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
Quantity:  5μg/20μg/1000μg
AA Sequence:  APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL
 PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF
 ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay usingmurine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of＞ 5.0
 × 106 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rMuIL-2 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Interleukin-2
Mature mouse Interleukin-2 (IL-2) shares 56% and 73% amino acid (a.a.) sequence identity with human and rat IL-2,
respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and
human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in
varying preformed complexes. The 55 kDa IL-2 Rαis specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which
is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chainγc/IL-2 Rγ,
which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding,
signal transduction is performed by both IL-2 Rβandγc. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rα
synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+
memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the
development of Th17 polarized cells.