Catalog Number:  C22-126-06
Source:  Escherichia coli.
Molecular Weight:  Approximately 15.6 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
Quantity:  20μg/100μg/1000μg
AA Sequence:  MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL
 KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL
 LPESSLRKRK RSRC
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by an anti-viral assay
 using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of＞ 1.3 × 106 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4, containing 5% trehalose.
Endotoxin:  Less than 1EU/μg of rMuIFN-γ as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Murine Interferon-gamma
Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-
lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins.
Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only
in human and primate cells.IFN-γ was originally characterized based on its antiviral activities. The protein also exerts
antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ
induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte
adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of
immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.