Recombinant Rat Fibroblast Growth Factor-basic
(rRtbFGF )
Catalog Number: C22-144-02
Source: Escherichia coli.
Molecular Weight: Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 145 amino acids.
Quantity: 10μg/50μg/1000μg
AA Sequence: PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG
VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKYSSWYVALKRT
GQYKLGSKTG PGQKAILFLP MSAKS
Purity: >98% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
assay using murine NR6R/3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of> 5.0 × 106 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.
Endotoxin: Less than 1EU/μg of rRtbFGF as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Rat Fibroblast Growth Factor-basic
Fibroblast Growth Factor-basic (bFGF) is one of 23 known members of the FGF family. Proteins of this family play a central role
during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and
differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney,
retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c,
2c, 3c and 4.