產(chǎn)品名稱 :Abcam Human CA125 ELISA 試劑盒 (MUC16) SimpleStep (ab195213)
Abcam Human CA125 ELISA 試劑盒 (MUC16) SimpleStep (ab195213)產(chǎn)品概述：
Abcam’s CA125 (MUC16) in vitro SimpleStep ELISA? (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human serum, plasma, cell culture supernatants, urine, milk, and saliva samples.
The SimpleStep ELISA? employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Abcam Human CA125 ELISA 試劑盒 (MUC16) SimpleStep (ab195213)存放說明：
Store at +4°C. Please refer to protocols.
Abcam Human CA125 ELISA 試劑盒 (MUC16) SimpleStep (ab195213)種屬反應(yīng)性：
與反應(yīng): Human
Abcam Human CA125 ELISA 試劑盒 (MUC16) SimpleStep (ab195213)組件：
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組件
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1 x 96 tests
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10X CA125 (MUC16) Capture Antibody
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2 x 300μl
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10X CA125 (MUC16) Detector Antibody
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2 x 300μl
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10X Wash Buffer PT (ab206977)
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1 x 20ml
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50X Cell Extraction Enhancer Solution (ab193971)
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1 x 1ml
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5X Cell Extraction Buffer PTR (ab193970)
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1 x 10ml
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Antibody Diluent CPI - HAMA Blocker (ab193969)
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2 x 3ml
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CA125 (MUC16) Human Lyophilized Recombinant Protein
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2 vials
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Plate Seals
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1 unit
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Pre-Coated 96-Well Microplate
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1 unit
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Sample Diluent 50BS
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2 x 12ml
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Sample Diluent NS (ab193972)
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1 x 12ml
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Stop Solution
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1 x 12ml
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TMB Substrate
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1 x 12ml
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