Recombinant Human Interleukin-1 
                                    receptor antagonist (rHuIL-1RA）

Catalog Number:  C22-101-01C
Source:  Escherichia coli.
Molecular Weight:  Approximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Quantity:  20μg/100μg/1000μg
AA Sequence:  MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVVPIEPHALFLG
 IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW
 FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by inhibiting IL-1α-
 dependent proliferation of murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of＞ 2.0 × 106 IU/mg in the presence of 50pg/ml rHuIL-1α. Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rHuIL-1RA as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.