Recombinant Human Interleukin-2
(rHuIL-2)
Catalog Number: C22-101-02
Source: Escherichia coli.
Molecular Weight: Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
Quantity: 10μg/50μg/1000μg
AA Sequence: MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL
EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN
RWITFCQSII STLT
Purity: >97% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
assay using murine CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of＞ 1.0
× 107 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.5.
Endotoxin: Less than 1EU/μg of rHuIL-2 as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Human Interleukin-2
Interleukin-2 is a protein that has a variety of immunologic functions, the most notable being the ability of IL-2 to promote the
proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows an
approximately 60% sequence similarity, and human sequence IL-2 has been found to be active on murine cell lines. Some of the
biological activities attributed to IL-2 include:
• induction of secretion of interferon-γ and tumor necrosis factors -α and -β from peripheral blood mononuclear cells
• stimulation of the rate of synthesis of c-myc RNA and transferrin receptor
• activation of neutrophils
• stimulation of proliferation and maturation of activated helper T cells
• stimulation of proliferation of activated and natural killer cells and tumor-infiltrating lymphocytes, as well as enhancementof the ability of these cytotoxic lymphocytes to kill target cells
• induction of the expression of IL-2 receptors on T cells
• stimulation of proliferation of antibody-producing B cells
