Recombinant Human Bone Morphogenetic Protein-7
(rHuBMP-7)
Catalog Number: C22-108-07
Source: Escherichia coli.
Molecular Weight: Approximately 15.6 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids.
Quantity: 2μg/10μg/1000μg
AA Sequence: STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL
GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP
KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH
Purity: >95% by SDS-PAGE and HPLC analyses.
Biological Activity: Data is not available.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.
Endotoxin: Less than 1EU/μg of rHuBMP-7 as determined by LAL method.
Applications:
1. Molecular standard (Western, ELISA) in studying secreted BMP-7;
2. Preparing antibodies for BMP-7 monomer;
3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be
apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in
appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Human Bone Morphogenetic Protein-7
Human Bone Morphogenetic Protein-7 (BMP-7) is one of at least 15 structurally and functionally related BMPs, which are
members of the transforming growth factor β (TGF-β) superfamily. BMPs were originally identified as protein regulators of
cartilage and bone formation. However, they havesince been shown to be involved in embryogenesis and morphogenesis of
various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of
various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. BMP-7 is synthesized
as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two
identical proteins or a heterodimer of two related bone morphogenetic proteins.
