Recombinant Human Osteoprotegerin/Fc Chimera
(rHuOPG/Fc)
Catalog Number: C22-108-08
Source: Pichia. Pastoris.
Molecular Weight: Recombinant OPG/Fc contains 412 amino acid residues, including 180 residues from mature OPG
(a.a 22-201) and 232 residues from the Fc protein of human IgG1 (calculated molecular mass is 46.5
kDa). As a result of glycosylation, the recombinant OPG/Fc migrates as a 49 kDa protein in SDS-
PAGE under reducing conditions.
Quantity: 10μg/50μg/1000μg
AA Sequence: OPG 22-201: ETFPPKYLHY DEETSHQLLC DKCPPGTYLK QHCTAKWKTV CAPCPDHYYT
DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH
RSCPPGFGVV QGTPERNTVC KRCPDGFFSN ETSSKAPCRK HTNCSVFGLL
LTQKGNATHD NICSGNSEST QKCGIDVTL
Fc232: EPKSSDKTHT CPPCPAPEFE GAPSVFLFPP KPKDTLMISR TPEVTCVVVD
VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN
GKEYKCKVSN KALPTPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL
TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS
RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
Purity: >90% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The specific activity is determined by
neutralizing the stimulation ofU937 cellsin the presence of 10 ng/ml soluble RANKL.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1EU/μg of rHuOPG-Fc as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
-70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further
evaluation purposes. NOT FOR HUMAN USE.
Human Osteoprotegerin
Osteoprotegerin (OPG) is a member of the TNFR superfamily that can act as a decoy receptor for RANKL. Binding of soluble
OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells,
thereby leading to excess accumulation of bone and cartilage. OPG is expressed in a wide variety of tissues including ***** heart,
lung, kidney, liver, spleen, prostate, lymph node and bone marrow. OPG is secreted both as a monomeric and a dimeric protein. Its
primary structure consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR
proteins and constitutes the soluble OPG.
