Catalog Number:  C22-109-01
Source:  Escherichia coli.
Molecular Weight:  Approximately 5.0 KDa, a single non-glycosylated polypeptide chain containing 47 amino acids.
Quantity:  5μg/20μg/1000μg
AA Sequence:  GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK
Purity:  >98% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay
 usingCD34+ dendritic cells is less than 1000 ng/ml, corresponding to a specific activity of＞ 1.0 × 103 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.
Endotoxin:  Less than 1EU/μg of rHuBD-1 as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Human beta-Defensin 1
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm
of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds.
To date, four human β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. β-defensins are expressed on some
leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards
immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors and
are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. β-defensins
contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-Defensins are 3-5 kDa peptides ranging in size
from 33-47 amino acid residues.